Particle Segmentation from mixed background

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Joe Perkins
Joe Perkins on 27 Jun 2019
Commented: Joe Perkins on 28 Jun 2019
Hi all - I have these large images (this is just a snapshot) containing particles in resin. My aim is to create a binary mask to remove the background resin.
The problem I have encountered is that the resin (generally darker than the particles) contains pixels that are also in the range of those in the particles and also brighter (back lighting from microscope illumination).
So far I have tried,
  • edge detection (canny, sobel) but these still pick out some of the blemishes and then constructing a mask from the mash of lines is difficult
  • active contours (edge & chan-vese) but these are also unable to remove the brighter resin pixels effectively
  • lazy snapping - gets confused between foreground and background
Any help/pointers would be greatly apprechiated.
Many thanks,
Joe
  2 Comments
KALYAN ACHARJYA
KALYAN ACHARJYA on 28 Jun 2019
Edited: KALYAN ACHARJYA on 28 Jun 2019
Can you share a sample pic with pointing resin?
You have to remove resin from the images, yes?
Ofcourse, to create mask to segment no resin part.
Joe Perkins
Joe Perkins on 28 Jun 2019
Hi - there is already one attached. Called ‘mosaic’
Thanks

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