Image acquisition speed using a photometrics camera

I have an imaging camera that I wanted to use and so I developed a Matlab GUI for this camera. The GUI displays 16 bits 128x128 images acquired from the camera at a specific rate. To change and manipulate properties of the camera I am using the MMCore library. The image acquisition works smoothly (as I am only displaying and not saving the images). Overtime as the images are being acquired, the speed of the acquisition slows down significantly (even if I have a circular buffer).

The code used for the displaying the images is as follows:

import mmcorej.*; 
mmc = CMMCore; 
mmc.loadDevice('Camera', 'PVCAM', 'Camera-1') 
mmc.initializeDevice(mmc.getCameraDevice()); 
mmc.initializeCircularBuffer(); 
mmc.detectDevice(mmc.getCameraDevice()); 
mmc.setCircularBufferMemoryFootprint(1000); 
mmc.startContinuousSequenceAcquisition(500); 
cap = mmc.getBufferTotalCapacity(); 
img = zeros(128,128, 7000); 
sig = zeros(1, 7000); 
i=2; 
n=0; 
format shortg; 

while (mmc.isSequenceRunning()) 
    i=i+1; 
    if (mmc.getRemainingImageCount()> 0) 
        img1 = mmc.popNextImage; 
        [pixelType] = pixel(mmc); 
        [img1] = camprop(mmc, img1,pixelType); 
        img(:,:,i) = transpose(img1); 
        imagesc(img(:,:,i)); 
        sig(i) = img(50,50,i); 
        sig(2)=1500; 
        Y =[sig(i),sig(i-1)]; 
        X = [i , i-1]; 
        subplot(2,1,1); 
        plot(X, Y); 
        hold on; 
        drawnow;   
        subplot(2,1,2); 
        imagesc(img(:,:,i)); 
        drawnow; 
    end 
    if i== 1000 
        mmc.stopSequenceAcquisition(); 
    end 
end 

exposuretime = mmc.getExposure(); 
i 
mmc.clearCircularBuffer(); 
mmc.unloadAllDevices();

How can I make sure the frames will not slow down in Matlab?

Thank you in advance

[Carl]

Hi Ahmed, how does the memory usage change over time? You can monitor this with the 'memory' command in MATLAB, or with your OS task manager. If the memory usage is increasing, are you able to determine which commands correspond to the increase in memory? Additionally, how much memory does your system have? You are allocating 1000 MB to the buffer, you can try lowering this to see if it helps.

[ahmed niri]

Hi Carl, the memory usage does increase over time. Once the library is imported, the majority of memory gets used and then increases when we are acquiring more images. There is however enough memory for me to allocate 1000MB. Thanks

[Ali Özgür Argunsah]

Did you solve this problem?