What does the error, "fl:filesy​stem:Acces​sDenied" mean when trying to use Python?

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I am trying to call Python from MATLAB, but whenever I run any Python commands, I get the error: fl:filesystem:AccessDenied
For example:
>> pyenv
fl:filesystem:AccessDenied
>> py.list({'This','is a','list'})
fl:filesystem:AccessDenied
I can't find any information about what this means.
I have Python 3.9 installed and I can call Python from the Windows command line.
  3 Comments
Matt Butts
Matt Butts on 3 Apr 2024
I only have one MATLAB session open and I tried restarting my machine.I am also not getting anything additional for the error so I am not sure this is the same issue.
Matthias Schwaiger
Matthias Schwaiger on 12 May 2025
The same happens on my machine when editing simulink models (I do not use python). I use a separate prefdir and also a separate temp-folder for that Matlab instance only. Is there any way to see which file can not be accessed in order to track it down?

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Accepted Answer

Matt Butts
Matt Butts on 3 Apr 2024
I explicitly set the version to use and that resolved the issue.
>> pyenv(Version="C:\Users\gk874\AppData\Local\Programs\Python\Python39\pythonw.exe")
ans =
PythonEnvironment with properties:
Version: "3.9"
Executable: "C:\Users\gk874\AppData\Local\Programs\Python\Python39\pythonw.exe"
Library: "C:\Users\gk874\AppData\Local\Programs\Python\Python39\python39.dll"
Home: "C:\Users\gk874\AppData\Local\Programs\Python\Python39"
Status: NotLoaded
ExecutionMode: InProcess
To MathWorks Support, I think this error message could be greatly improved to help a user understand exactly what was happening. I expected MATLAB to find my Python installation directly, but that seems to not be the case.

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