ans =
From the Tips section in the documentation page -
"assume removes any assumptions previously set on the symbolic variables. To retain previous assumptions while adding an assumption, use assumeAlso."
syms Phi_geod(t) lambda_geod(t)
syms Phi_dot_geod(t) lambda_dot_geod(t)
assumptions(Phi_geod)
assume(Phi_geod(t),"real");
assumptions(Phi_geod)
assumeAlso(lambda_geod(t),"real");
assumptions(Phi_geod)
assumeAlso(Phi_dot_geod(t),"real");
assumeAlso(lambda_dot_geod(t),"real");
assumptions(Phi_geod)